Cell Models

Primary Cell Specialists

  • Primary human cells from a wide range of tissues, all produced with full ethical permission
  • Catalogued cell products or customized client-specific cell production
  • Disease and donor-specific requirements can be accommodated
  • Primary animal cells from domesticated species, for agri-bio and veterinary studies

 

3D cell culture models

  • Application of scaffold technologies to create 3D cell models in 96-well formats
  • 3D models incorporating electrospun scaffolds or hydrogels
  • Assay readouts using standard fluorescent readouts or flourescence-based cell population methods
  • Novel cell-based assembly via bio-printing and additive printing (See Cell Product Manufacturing)

Cell cryopreservation

  • Cell freezing in situ in multiwell plates using AvantiCell proprietary technology
  • 2D cell models delivered in frozen, “plug and play” formats.
  • User-friendly 2D cryopreservation technology applicable to human primary cells, iPSC-derived cells with differentiated function and cell lines

Cell product manufacturing

  • Novel methods for assembly of conventional 2D and complex 3D cell models
  • Innovative additive manufacturing of advanced 3D models for toxicity testing developed under EU Horizon 2020 SME Instrument Phase 2 Project “ANAPRINT”
  • Innovative bio-printing of advanced 3D cell models for neurodegenerative drug discovery developed under EU Horizon 2020 Fast Track to Innovation Project “CENSUS”
  • When combined with AvantiCell proprietary in situ cell cryopreservation, additive/bio-printing techniques deliver scalable manufacturing of 3D cell models with high preclinical predictive value

Mucosal barrier tissue models

  • Cell models reconstituting mucosal immunity / inflammation in human barrier tissues
  • Co-culture models incorporating lung/intestinal processing/transport modules, all based upon human primary cells
  • Intestinal model for study of metabolic processing and biological impact of ingested materials, based upon intestinal/colonic endogenous progenitors
  • Lung model for study of fate of inhaled therapeutics or atmospheric pollutants
  • Second co-cultured cell type selectable according to disease target
  • Disease targets include metabolic disease (liver, muscle, adipose cell types) and inflammation (immune cell types)

Nanosafety

  • Cell-based models with proven ability to detect and evaluate nano-object biosafety
  • Acute toxicity screening model uses haemocyte cells from the marine mussel Mytilus, a sentinel species
  • Lung air:liquid interface model to evaluate aerosol or airborne environmental samples
  • Proprietary tool for capture and presentation of environmental samples to cell screening models (EU Horizon 2020 ETN project “PANDORA”, under development)

Processes

Printing

  • Additive printing / bio-printing for customized 3D system assembly
  • Scaffold printed directly into culture wells
  • Cells printed or guided directly into scaffolds
  • Novel printed medium-throughput cell products

Bio-manufacturing

  • Bio-manufacturing processes for 2D/3D cell-based analysis system production
  • Cryopreservation technology for in situ multiwell cell freeze-thaw

Enabling Technologies

  • Cell cryopreservation
  • Magnetic cell guiding

R & D

Technologies have been developed within AvantiCell through strategic R&D.
R&D programmes supported by £8.6M of competitively-awarded funding.